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Sample submission
Sample submission
The NMR instrument in teaching labs is run as a submission service. The following 1D and 2D experiments are available as routine experiments. In addition, non-standard experiments can be recorded if required. For example increased scans or variable temperature experiments. Where specialist experiments are not available within teaching labs, samples will be transferred to other service instruments in the department.
Taught Undergraduates
Taught Undergraduates
Samples should be dissolved in the minimal amount of deuterated solvent in a sample vial and be filtered through cotton wool into a clean NMR tube. Please be careful with the tubes, they are expensive and can be reused many times. Please return any dirty or damaged tubes to the prep-room for washing and reuse.
Solvents available
Solvents available
Deuterated chloroform (preferred)
Deuterated water
Other solvents may be available in exceptional circumstances, please discuss your requirements with the technical staff.
Computer submission
Computer submission
Samples should be labelled with one of the numbered tags and be placed in the appropriate pot on the bench opposite the prep room. The details for your sample and the experiments you require should be filled in on the google form accessed by the submission hub.
Your sample will be taken to be queued up on the spectrometer and is usually run overnight.
Teaching Laboratory Based Project students
Teaching Laboratory Based Project students
When projects are running in the lab (eg BSc and MChem miniprojects), we operate a prioritisation system for samples in order to ensure fast turn-around of results.
During the day only samples requesting a single 1D proton, phosphorous or fluorine experment will be submitted during the day in order to provide a rapid return of results. All other samples will be queued to run overnight.
If you would like multiple experiments on a sample (eg carbon, DEPT, COSY, HSQC), it is good practice to record and view the results for a short experiment (proton, phosphorous or fluorine) first and ensure that your sample is suitable for recording additional experiments in order to minimise wasted spectrometer time. All samples for project students will be retained until 4 pm on the following Tuesday and you should request additional experiments using the submission form. You should not generally prepare new samples for recording additional nuclei.
If the nature of your chemistry will require samples to be run immediately after they have been prepared (whether for short or long experiments), please liase with the technical staff as early as possible, and certainly in advance of any sample preparation. Wherever possible we attempt to assist with these requests.
Please discuss any NMR requirements with the demonstrators in the labs, the technical staff or your project supervisor for advice.
Other users
Other users
Access to NMR for any other category of users is via the departmental NMR service. Please contact the NMR service technicians to discuss your requirements.
Available experiments
Available experiments
1D Experiments
1D Experiments
Experiment
Experiment
PPM range
PPM range
Default scans
Default scans
Acquisition time
Acquisition time
H Service
0 to +10
16
5 min
H Acid
0 to +18
16
5 min
Hydride
-20 to +15
16
5 min
C Service
0 to +220
256
15 min
C+DEPT
0 to +200
C-13 with DEPT-135 overlay
256/64
20 min
DEPT
0 to +200 DEPT-90 and DEPT-135
64/64
10 min
C Wide
0 to +400
256
15 min
P Service
-10 to +80
32
5 min
P Wide
-200 to +200
16
5 min
F Service
-200 to +200
16
5 min
Si Service
-30 to + 20
256
25 min
2D NMR experiments
2D NMR experiments
Experiment
Experiment
PPM range
PPM range
Default scans
Default scans
Acquisition time
Acquisition time
H-H COSY
0 to +10
2
10 min
H Service
H: 0 to 10
C: 0 to 220
2
15 min
H Service
H: 0 to 10
C: 0 to 220
8
60 min
H Service
H: –10 to 10
P: 0 to 100
2
20 min
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