Jenway 7415 Spectrometers
Instrument
Teaching Labs has six Jenway 7415 single-beam photometers. These are best used for photometric or kinetic measurements, although they are also capable of recording scanning spectra (but you are advised to use dual beam instruments instead).
Specification
Beam type
Split
Cuvette size
Standard 1 cm pathlength cuvettes
Wavelength range
1000 - 198 nm (cuvette dependent)
Maximum resolution
1 nm
Wavelength accuracy
± 2 nm
Photometric limits
–0.3 to + 2.5 Abs
Preferred photometric range
0 to 1.5 Abs
Temperature range (with Peltier)
+20 to +50 °C
Use
Scanning Mode
The Jenway 7415 photometers can record scanning spectra, however better results can be obtained from a dual-beam instrument. Scanning spectra are often recorded in order to determine the wavelength of maximum absorbance (λmax) which can then be used for photometric or kinetic measurements, or to provide characterisation data for a compound.
Photometric Mode
Photometric mode is used to record absorbance values at a given wavelength. As absorbance is related to concentration through the Beer-Lambert law (A = ε c l), the concentration of a solution can be determined through the use of standards and recording a set of measurements in photometric mode.
Kinetics Mode
Kinetic mode is used to record the absorbance at a given wavelength as a function of time. This allows the study of reaction rates for reactions which have starting material(s) or product(s) having an absorbance within the range of the spectrophotometer.
Instructions
Photometrics
Jenway 7415 Photometrics (PDF) ↓
Kinetics
Spectrum
Please contact a technician for training.
Accessing data
Data is available in the UVJ folders.
Filenames
The default naming scheme for files is as follows:
Data type
Filename
Photometric
Photometric_YYYYMMDD_HHMMSS
Kinetics
Kinetics_YYYYMMDD_HHMMSS
Spectrum
Spectrum_YYYYMMDD_HHMMSS