Using pipettes

Insert the top of a pipette into a pipette filler. The pipette should only be inserted a short way into the pipette filler, only enough to form a seal (usually only a few mm). Pushing the pipette in too far is likely to damage the pipette filler by dislodging valves in the fillers.

Squeeze valve A allowing the bulb to be emptied of air and deflate the bulb.

Place the tip of the pipette into the liquid to be drawn up and carefully squeeze the fill valve (S) which will draw the liquid up the pipette. The amount of pressure applied to the fill valve will vary the speed at which the liquid is drawn up. Continue to draw up liquid until the desired graduation mark is reached.

If excess liquid is drawn up, use the empty valve (E) to slowly release some liquid until the correct quantity is reached.

Transfer the pipette from the liquid and move it to the target vessel. Keep the pipette vertical and position the two vessels as close to each other as practical.

Release the liquid by squeezing the empty valve (E). For non-blowout pipettes, keep squeezing the empty valve and touch the tip of the pipette to the liquid surface allowing the surface to draw the liquid from the pipette leaving only the drop in the tip behind. For blowout pipettes, ensure the remaining drop is added to the collection flask.