Fluorimetry

Introduction to Fluorimetry

Spectrofluorimeters are able to record excitation and emission spectra for solutions.

Cuvettes

Fluorescence cuvettes have four optical faces as emission is recorded perpendicular to the excitation source in the instrument. Cuvettes are available in the same range of materials as UV-Vis spectroscopy including: polystyrene (PS); polymethyl methacrylate (PMMA); Brand-UV; and quartz. Cuvettes are only available with a 1 cm pathlength and 4 mL sample volume. For most fluorescence spectroscopy Brand-UV cuvettes are recommended as the first choice.

Instrumentation

Legacy