Agilent
Instrument
Teaching Labs has four Agilent HPLC systems which allow separation and quantification of components within a solution. These instruments are used as a service with students submitting samples to be placed on the autosamplers.
Pump Specification
Model
1260 Infinity II Quaternary Pump
Flow range
0.2 - 10 mL/min
Injection Range
0.1 - 100 µL
System Pressure Operating Range
Up to 600 bar
Detector Specification
Model
1260 Infinity II Multiple Wavelength Detector
Wavelength range
190 - 950 nm (8 possible wavelength analysis)
Wavelength accuracy
± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
Light source
Deuterium and tungsten lamps
Flow cell volume
13 µL
Cell Path Length
10 mm
Maximum Pressure
120 bar (1740 psi)
Instructions
Samples for HPLC analysis on the agilents are submitted to the chromatography service.
Sample preparation
Samples should be submitted in screw-top HPLC vials which are available from the HPLC submission points around the lab. Sample vials need to be filled to at least 50% depth in order for the injection needle to be able to draw up sample.
Computer submission
Samples should be labelled with a HPLC label containing a submission number and be placed in the appropriate tray on the bench opposite the prep room. The details for your sample and the experiments you require should be filled in on the google form accessed by the submission hub. Your samples will be queued up on the autosampler by a technician and the results will usually be available the next day.
Accessing data
Chromatograms are returned electronically. In order to locate your data, you will need to identify the date your spectra were submitted and which instrument your sample has been recorded on in the HPLC submission form below.
Results are then accessible via FTP from ftp://chemtl.york.ac.uk/HPLC-Agilent/. Select the instrument used to record your chromatogram, then the file type required and then locate your data. Files are named with the date and time the sample was recorded (yyyymmdd hhmmss) followed by the sample number (eg HPLC00001).
Current Results
Standards
In addition to your data, standards are often recorded to aid interpretation of chromatograms. These will be stored in the same folders (Instrument/file type/date) and will have been recorded in the same sequence as your sample. Standards will be clearly labelled and should be used to aid identifying components by matching retention times, usually named with the date/time the sample was recorded then details of the standard.
File formats available
Data is available in the following formats:
PDF
Portable document format. An auto processed document containing the chromatogram and automatically generated peak table with integrations.
CH
Chromatogram outputs for each wavelength used in detection. This format can be imported directly into ACD/Spectrus for reprocessing.
TXT
A text summary of peaks identified.
CSV
The individual datapoints making up the chromatogram with time in seconds and voltage in milliamps.
CDF
Agilent CDS format.