Pump Specification

Detector Specification

Instructions  

Samples for HPLC analysis on the agilents are submitted to the chromatography service.

Sample preparation

Samples should be submitted in screw-top HPLC vials which are available from the HPLC submission points around the lab. Sample vials need to be filled to at least 50% depth in order for the injection needle to be able to draw up sample.

Computer submission

Samples should be labelled with a HPLC label containing a submission number and be placed in the appropriate tray on the bench opposite the prep room. The details for your sample and the experiments you require should be filled in on the google form accessed by the submission hub. Your samples will be queued up on the autosampler by a technician and the results will usually be available the next day.

Accessing data

Chromatograms are returned electronically. In order to locate your data, you will need to identify the date your spectra were submitted and which instrument your sample has been recorded on in the HPLC submission form below.

Data is found in the HPLC-Agilent folder which can be accessed using the Accessing Data guides. In this folder you will then need to select the instrument used to record your chromatogram, then the file type required and then locate your data.

Standards

In addition to your data, standards are often recorded to aid interpretation of chromatograms. These will be stored in the same folders (Instrument/file type/date) and will have been recorded in the same sequence as your sample. Standards will be clearly labelled and should be used to aid identifying components by matching retention times, usually named with the date/time the sample was recorded then details of the standard.